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KMID : 0377619890540120831
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Korean Jungang Medical Journal
1989 Volume.54 No. 12 p.831 ~ p.837
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Change of Amino Acid Levels in Renal Tissues of Rabbits
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Abstract
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Several organic solutes -have been identified as osmotically active organic solutes or osmolytes in the medulla of mammalian kidney.
Because high levels of inorganic salts and urea inhibit various cellullar enzymes, non-. perturbing organic solutes are more proper than perturbing solutes for adaptation to high salt environment. Among these osmolytes, polyols, methylamines and amino acids are found in the medulla of mammalian kidney.
Even though there is no direct evidence, some authors suggest that amino acids play a role of osmolytes in the mammalian kidney. The kinds and metabolism of osmolytes are very complex. Also variables. such as sample preparation and detection method and experimental condition deviate the true levels of osmolytes in the kidney.
In an attempt to clarify the role of amino acids as osmolytes in the rabbit kidney and to select the proper time of the sample preparation for amino acids, we measured the levels of amino acids in rabbit kidney tissues with time course.
The concentrations of taurine, aspartic acid, glutamine, serine and alanine in the medulla were above 2 mM/kg wet weight and higher than in the cortex. Those levels in the medulla were decreased gradually with time course, but those in the cortex were increased gradually.
Of amino acids in rabbit kidney, the concentrations of glycine, the highest one, in the medulla and cortex were 5.89 ± 2.44 mM/kg wet wt and 5.56 ± 1.12 mM/kg wet wt respectively. With time course, glycine level in the cortex was increased to 7.54 ± 3.95 mM/kg wet wt. p
The concentrations of valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine and tryptophan in the kidney tissues were below 1 mM/kg wet wt.
Lysine and arginine did not show special patterns.
The results of this experiment show that amino acids in rabbit kidney play a role as osmotically active organic solutes and the sample has to be prepared immediately after the extraction of kidney for amino acid detection.
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